alexa fluor 488 Search Results


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Servicebio Inc alexa fluor
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Novus Biologicals alexa flour 488 conjugated mouse anti gfap
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R&D Systems cldn6 alexa 507 fluor 488 conjugated antibody
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novus biologicals nb300-144af488
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Novus Biologicals anti human vimentin antibody
Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of <t>vimentin</t> staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.
Anti Human Vimentin Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems cd279
Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of <t>vimentin</t> staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.
Cd279, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc alexa fluor 488
Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of <t>vimentin</t> staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.
Alexa Fluor 488, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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Jackson Immuno alexafluor 488
Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of <t>vimentin</t> staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.
Alexafluor 488, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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Jackson Immuno goat anti rabbit alexa fluor 488
Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of <t>vimentin</t> staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.
Goat Anti Rabbit Alexa Fluor 488, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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Jackson Immuno donkey α rabbit 488
Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of <t>vimentin</t> staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.
Donkey α Rabbit 488, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno alexa fluor 488 conjugated affinepure f ab 2 fragment goat anti mouse igg igm
Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of <t>vimentin</t> staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.
Alexa Fluor 488 Conjugated Affinepure F Ab 2 Fragment Goat Anti Mouse Igg Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno alexa fluor 488 affinipure goat anti rat igg
Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of <t>vimentin</t> staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.
Alexa Fluor 488 Affinipure Goat Anti Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of vimentin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.

Journal: Advanced Biology

Article Title: Mechanical Loading of Osteocytes via Oscillatory Fluid Flow Regulates Early‐Stage PC‐3 Prostate Cancer Metastasis to Bone

doi: 10.1002/adbi.202400824

Figure Lengend Snippet: Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of vimentin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.

Article Snippet: Cells were then incubated with a conjugated primary mouse anti‐human vimentin antibody (Novus Biologicals, NBP1‐92687AF488) at a 1:100 dilution in PBS and 1% BSA overnight at 4 °C in the dark, and counterstained with 4',6‐diamidino‐2‐phenylindole (DAPI) at a 1:1000 dilution at room temperature for 10 min.

Techniques: Marker, Expressing, Staining, Fluorescence